Activating Compound | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Chromobacterium violaceum |
Cloned (Comment) | Organism |
---|---|
gene vioD, recombinant expression in Escherichia coli strain DH10B and Streptomyces albus strain J1074, coexpression with the other genes of the violacein biosynthesis pathway, reevaluation, overview | Chromobacterium violaceum |
Protein Variants | Comment | Organism |
---|---|---|
additional information | additional coexpression of vioC and/or vioD in Streptomyces albus. Disruption of vioA or vioB completely abrogates the biosynthesis of violacein intermediates, while disruption of vioC or vioD results in the production of violacein precursors | Chromobacterium violaceum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Chromobacterium violaceum | VioD is no tryptophan 5-hydroxylase | ? | - |
? | |
additional information | Chromobacterium violaceum JCM 1249 | VioD is no tryptophan 5-hydroxylase | ? | - |
? | |
protodeoxyviolaceinate + NAD(P)H + H+ + O2 | Chromobacterium violaceum | - |
protoviolaceinate + NAD(P)+ | - |
? | |
protodeoxyviolaceinate + NAD(P)H + H+ + O2 | Chromobacterium violaceum JCM 1249 | - |
protoviolaceinate + NAD(P)+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chromobacterium violaceum | Q9S3U8 | strain JCM1249 or ATCC12472 | - |
Chromobacterium violaceum JCM 1249 | Q9S3U8 | strain JCM1249 or ATCC12472 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | VioD is no tryptophan 5-hydroxylase | Chromobacterium violaceum | ? | - |
? | |
additional information | VioD is no tryptophan 5-hydroxylase | Chromobacterium violaceum JCM 1249 | ? | - |
? | |
protodeoxyviolaceinate + NAD(P)H + H+ + O2 | - |
Chromobacterium violaceum | protoviolaceinate + NAD(P)+ | - |
? | |
protodeoxyviolaceinate + NAD(P)H + H+ + O2 | - |
Chromobacterium violaceum JCM 1249 | protoviolaceinate + NAD(P)+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
VioD | - |
Chromobacterium violaceum |
VioD oxygenase | - |
Chromobacterium violaceum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Chromobacterium violaceum | |
NADPH | - |
Chromobacterium violaceum |
General Information | Comment | Organism |
---|---|---|
evolution | VioD belongs to the family of flavin-containing NAD(P)H-dependent monooxygenases | Chromobacterium violaceum |
malfunction | disruption of vioA or vioB completely abrogates the biosynthesis of violacein intermediates, while disruption of vioC or vioD results in the production of violacein precursors. Gene vioE is essential for violacein biosynthesis. Enzymes VioC and VioD are responsible for the oxygenations at the ox-indole and hydroxyindole, respectively. In the absence of VioC, the expected proviolaceins are accompanied by other compounds: deoxychromoviridans, chromoviridans, and oxychromoviridans | Chromobacterium violaceum |
metabolism | vioD is one gene encoding violacein biosynthesis, VioD appears to act on late steps of violacein biosynthesis, overview | Chromobacterium violaceum |